Effect of MMP-28 antisense oligodeoxynucleotide on biological behaviour of human lung cancer cell line A549 / 基础医学与临床

ABSTRACT

Objective To study the effect of matrix metalloproteinase(MMP)-28 antisense oligodeoxynucleotide(AODN) on cell biological behaviour of human lung cancer cell A549.Methods To explore its possible functions,cell line A549 was subjected to specific inhibition of MMP-28 by AODN transfection.RT-PCR and Western blot were used to evaluate mRNA and protein level of MMP-28.The proliferation status of cells was measured by MTT assay and soft-agar colony formation assay.Cell invasion ability was detected by Matrigel in vitro invasion assay.The ability of tumor formation and of metastatic potency was examined by lung cancer cells xenograft in nude mice.Results Forty-eight hours after transfection,the expression of MMP-28 mRNA and protein in AODN-transfected cells was significantly lower than those of control cells and SODN-transfected cells(P