Cellular Mechanism of Newly Synthesized Indoledione Derivative-induced Immunological Death of Tumor Cell
Immune Network
;
: 383-389, 2011.
Article
in English
| WPRIM
| ID: wpr-60135
ABSTRACT
BACKGROUND:
EY-6 is one of the newly synthesized indoledione derivatives to induce tumor cell-specific cell death. In this study, we investigated the mechanism of immunological death induced by EY-6 at mouse colon cancer cell as well as at the normal immune cell represented by dendritic cell.METHODS:
C57BL/6 mouse syngeneic colon cancer cell MC38 was treated with EY-6, and analyzed by MTT for viability test, flow cytometry for confirming surface expressing molecules and ELISA for detection of cytokine secretion. Normal myeloid-dendritic cell (DC) was ex vivo cultured from bone marrow hematopoietic stem cells of C57BL/6 mice with GM-CSF and IL-4 to analyze the DC uptake of dead tumor cells and to observe the effect of EY-6 on the normal DC.RESULTS:
EY-6 killed the MC38 tumor cells in a dose dependent manner (25, 50 and 100 microM) with carleticulin induction. And EY-6 induced the secretion of IFN-gamma but not of TNF-alpha from the MC38 tumor cells. EY-6 did not kill the ex-vivo cultured DCs at the dose killing tumor cells and did slightly but not significantly induced the DC maturation. The OVA-specific cross-presentation ability of DC was not induced by chemical treatment (both MHC II and MHC I-restricted antigen presentation).CONCLUSION:
Data indicate that the EY-6 induced tumor cell specific and immunological cell death by modulation of tumor cell phenotype and cytokine secretion favoring induction of specific immunity eliminating tumor cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phenotype
/
Bone Marrow
/
Dendritic Cells
/
Enzyme-Linked Immunosorbent Assay
/
Hematopoietic Stem Cells
/
Granulocyte-Macrophage Colony-Stimulating Factor
/
Interleukin-4
/
Tumor Necrosis Factor-alpha
/
Cell Death
/
Colonic Neoplasms
Limits:
Animals
Language:
English
Journal:
Immune Network
Year:
2011
Type:
Article
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