Exosome-derived miR-20a inhibit apoptosis of TAM by targeting BCL2L11 in nasopharyngeal carcinoma / 重庆医学
Chongqing Medicine
;
(36): 721-724,728, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-606307
ABSTRACT
Objective To investigate whether exosome-derived microRNA of nasopharyngeal carcinoma suppresses apoptosis of tumor associated macrophage (TAM).Methods Target microRNAs and genes were determined by bioinformatics methods.Isolated exosomes were used to detect miR-20a expression by qRT-PCR.Furthermore,apoptosis index and proteins involved in apoptotic pathways were detected after miR-20a mimic and inhibitor transfection into macrophages.Results miR-20a expression was upregulated in isolated exosomes.miR-20a target gene was BCL2L11.MiR-20a overexpression could inhibit apoptosis of macrophages,meanwhile,apoptotic pathways related proteins Bim,caspase-9 and caspase-3 were significantly suppressed by miR-20a mimic(P<0.05).Condusion miR-20a can suppress activation of Bim-caspase-9-casepase-3 and resulting in apoptotic inhibition of macrophages.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chongqing Medicine
Year:
2017
Type:
Article
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