Ganoderma lucidum extract improves immune function in Walker-256 ascites rats / 第二军医大学学报

ABSTRACT

Objective To explore the effect of Ganoderma lucidum extract (GLE) on immune function of Walker256 ascites rats and its mechanisms.Methods Sixty Wistar rats were randomly divided into 6 groupscontrol group,model group,high-dose GLE group,medium-dose GLE group,low-dose GLE group and cisplatin group according to different treatments,with 10 rats in each group.After the Walker-256 ascites rat model was successfully established,the rats in the low-dose,medium-dose and high-dose GLE groups were intragastrically administered with 0.84,1.68 and 3.36 g/(kg · d) GLE,respectively,twice a day,2 mL each time,for 14 days;the rats in the cisplatin group were intraperitoneally injected with 0.004 g/(kg · d) cisplatin in normal saline once a week;the rats in control group and model group were intragastrically administered with normal saline,2 mL each time,twice a day for 14 days.The general health status of the rats of each group were observed,the mass of ascites was tested,the spleen,thymus and kidney indexes were calculated,and the renal function was measured.Flow cytometry was used to measure the cell cycles of thymus and bone marrow cells,the distributions of T lymphocyte subsets and the number of NK cells in peripheral blood.Serum immune cytokine protein chip was used to measure the serum levels of immune cytokines of rots in the control group,model group and medium-dose GLE group.Results GLE significantly reduced the mass of ascites in rats (P＜ 0.05).Compared with the control group,thymus,spleen and kidney were damaged in the model group and cisplatin group,and treating rats with GLE significantly increased the thymus index and renal indexes,and significantly decreased serum creatinine and urea nitrogen (P＜0.05).G0/G1 phase arrest was significantly induced in the thymus and bone marrow cells in the model group and the cisplatin group (P＜0.05);and GLE significantly reduced the arrest of G0/G1 phase and significantly induced the transformation of thymus and bone marrow cells to G2/M and S phases (P＜0.05).GLE significantly increased the number of CD4+ cells,the ratio of CD4+ to CD8+ ceils and the number of NK cells,and significantly decreased the number of CD8+ cells in the peripheral blood of rats as compared with the model group (P＜0.05).Compared with the model groups,the expressions of interferon-γ (IFN-γ),interleukin (IL)-1α,IL-1β,IL-2,IL-4,IL-10 and tumor necrosis factor-α (TNF-α) in serum of the rats in medium-dose GLE group were significantly increased (P＜0.05).Conclusion GLE can effectively reduce the generation of ascites and improve immune function in Walker-256 ascites rats,and has no renal damage effect compared with cisplatin.