The effect of interleukin-17 on podocyte-associated factors and apoptosis and molecular mechanism / 中国小儿急救医学
Chinese Pediatric Emergency Medicine
; (12): 587-592, 2017.
Article
in Zh
| WPRIM
| ID: wpr-607154
Responsible library:
WPRO
ABSTRACT
Objective To investigate the effect of interleukin(IL)-17 on podocyte-associated factors and apoptosis in podocytes and explore the molecular mechanism.Methods The podocytes were used as the object of study.The apoptosis of podocytes induced by IL-17 in a dose(1nm/ml,10nm/ml,50nm/ml,100nm/ml) and time(12h,24h,48h,72h) way and the protein expression of Fas and FasL of podocytes induced by IL-17 were detected by flow cytometrey.The podocytes were divided into the blank control group and 100ng/ml IL-17 induction group.The expressions of mRNA of Nephrin,WT1,Synaptopodin,Podocylaxin,Fas and FasL were measured by Real-time RT PCR.The proteins of WT1,Caspases8,Caspases3 in podocyte were detected by immunocytochemistry method.Results IL-17 promoted the apopotosis of podocyte in a dose and time way(P<0.01),and increased the expression of Fas,Caspase 8 and Caspase 3 in podocyte(P<0.01).IL-17 decreased the expression ofPodocylaxin (P<0.05),but had no effect on Nephrin,WT1,Synaptopodin,and FasL in podocyte(P>0.05).Conclusion IL-17 may causerenal injure by inducing the apoptosis of podocyte and decreasing the expression of Podocylaxin in podocyte.
Full text:
1
Index:
WPRIM
Language:
Zh
Journal:
Chinese Pediatric Emergency Medicine
Year:
2017
Type:
Article