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Establishement of HPLC fingerprints of flavonoids in Ziziphi spinosae Semen and determination of two constituents / 中成药
Article in Zh | WPRIM | ID: wpr-609761
Responsible library: WPRO
ABSTRACT
AIM To establish the HPLC fingerprints of flavonoids in Ziziphi spinosae Semen and to determine the contents of two constituents.METHODS With spinosin as a reference peak,the HPLC fingerprints of ten batches of samples were established.The analysis of methanol extract of flavonoids in Ziziphi spinosae Semen was performed on a 30 ℃ thermostatic Agilent TC-C18column (4.6 mm × 250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1% acetic acid flowing at 1 mL/min in a gradient elution manner,and the detection wavelength was set at 280 nm.RESULTS There were ten common peaks in the HPLC fingerprints,eight of which (vicenin-Ⅱ,glucosylvitexin,isospinosin,spinosin,6'-pyridyloylspinosin,6'-p-hydroxybenzoylspinosin,6'-feruloylspinosin and 6'-p-coumaroylspinosin) were identified.Spinosin and 6'-feruloylspinosin showed good linear relationships within the ranges of 15.00-40.00 μg and 5.00-14.00 μg,whose average recoveries (RSDs)were 100.5% (1.6%) and 100.4% (1.6%),respectively.CONCLUSION This accurate,simple and reliable method can be used for the quality control of flavonoids in Ziziphi spinosae Semen.
Key words
Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Traditional Patent Medicine Year: 2017 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Traditional Patent Medicine Year: 2017 Type: Article