Carnosic Acid Cooperates with Tamoxifen to Induce Apoptosis of Breast Cancer Cells through Caspase-3 Signaling Pathway / 华中科技大学学报(医学版)

ABSTRACT

Objective To explore the effect of carnosic acid combined with tamoxifen on breast cancer cells and the mechanism.Methods Breast cancer cell lines MCF-7,T47D were divided into 4 groups:PBS was given to control group,carnosic acid(CA)was added to CA group,tamoxifen(TAM)was added to TAM group,and CA+TAM group was treated with carnosic acid and tamoxifen.The proliferation ability was measured by MTT assay,the colony formation capacity was measured by colony formation assay,the invasion ability was measured by Transwell chamber assay,the migration ability was measured by wound-healing assay,the expression of Caspase-8,Caspase-3 and PARP protein were measured by Western blotting.Results CA,TAM and CA+TAM suppressed breast cancer cell proliferation with a dose-dependent manner.Compared with CA group and TAM group,the proliferation inhibitory rate of CA+TAM group was significantly increased(P0.05).In two breast cancer cell strains,the expression of cleaved Caspase-8 protein between control,CA,TAM and CA+TAM groups was not significantly different(P>0.05).Compared to control group,the expression levels of cleaved caspase-3,cleaved PARP protein in CA,TAM and CA+TAM groups were significantly increased(P0.05).Conclusion Carnosic acid combined with tamoxifen can suppress the proliferation,invasion and migration of breast cancer cells,and its mechanism may be related to the activation of Caspase-3/PARP signaling pathway.