Identification of major constituents from propolis by LC-IT-TOF-MS / 中国药科大学学报
Journal of China Pharmaceutical University
;
(6): 178-183, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-613406
ABSTRACT
A qualitative analytical method of liquid chromatography coupled with ion trap time-of-flight mass spectrometry (LC-IT-TOF/MS) was developed for identification of major constituents in propolis from China (Shandong).The LC-IT-TOF-MS was performed on a Shim-pack VP-ODS column (2.0 mm× 150 mm,5 μm) with the mobile phase consisting of acetonitrile and water containing 0.2% formic acid in gradient mode,and the flow rate was set at 0.3 mL/min.Negative ion mode was used for IT-TOF-MS.According to the accurate molecular weight,MS fragment pathway,comparison with the retention time of reference compounds,total 31 compounds,including twenty-five phenolic acids and six flavonoids were identified.The LC-IT-TOF-MS qualitative analysis method can be used for analyzing major components of propolis quickly and accurately.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
/
Qualitative research
Language:
Chinese
Journal:
Journal of China Pharmaceutical University
Year:
2017
Type:
Article
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