Protection of silibinin sunscreen on chronic UVB induced guinea pig skin apoptosis / 国际中医中药杂志

ABSTRACT

Objective To study the protection of silibinin sunscreen on chronic UVB induced guinea pig skin apoptosis. Methods A total of 60 guinea pigs were randomly divided into four groups, blank group, UVB group, silibinin sunscreen group, anti-wrinkle day cream group (positive control), and 15 in each group. The UVB ultraviolet phototherapy instrument was used to irradiate guinea pig skin for 30 min once and twice per day, and then up to 15 days before the apoptosis of skin model has been set up. The silibinin sunscreen and anti-wrinkle day cream groups received silibinin sunscreen and anti-wrinkle day cream as intervetion, respectively. But the other two groups received nothing for intervention. The GSH-Px and SOD, and MDA kit were detected and compared. The westerrn blot detected caspase-3, Bax, Bcl-2 protein expression in each group. Results Compared with the UVB group, the contents of GSH-Px (8.79 ± 1.57 mg/g, 8.25 ± 2.07 mg/g vs. 4.66 ± 1.82 mg/g) and SOD (24.52 ± 5.43 U/mg, 27.81 ± 2.13 U/mg vs. 10.82 ± 3.40 U/mg) in the silibinin sunscreen group and anti-wrinkle day cream group increased significantly, and the content of MDA (7.91 ± 2.80 nmol/mg, 6.45 ± 1.86 nmol/mg vs. 13.57 ± 2.63 nmol/mg) decreased significantly (P<0.05, P<0.01). The expression ofcaspase-3 (0.44 ± 0.04, 0.38 ± 0.03 vs. 0.56 ± 0.06) and Bax (0.46 ± 0.05, 0.40 ± 0.06 vs. 0.75 ± 0.06) in the silibinin sunscreen group and anti-wrinkle day cream group decreased significantly, and the expression of Bcl-2 (0.39 ± 0.04, 0.43 ± 0.06 vs. 0.25 ± 0.05) increased significantly (P<0.05, P<0.01). Conclusions Protection of Silibinin Sunscreen on Chronic UVB Induced Guinea Pig Skin Apoptosis, mainly through enhancing GSH-Px and SOD activity and reducing MDA content, reducing the caspase-3, Bax protein expression and increasing the Bcl-2 protein expression.