Prevalence of Haplorchis taichui in Field-Collected Snails: A Molecular Approach
The Korean Journal of Parasitology
; : 343-346, 2010.
Article
in En
| WPRIM
| ID: wpr-62038
Responsible library:
WPRO
ABSTRACT
The prevalence of the cercarial stage of an intestinal trematode, Haplorchis taichui, in thiarid snails (Gastropoda Thiaridae) was investigated using light microscope and species-specific PCR procedures. A total of 988 snails were collected from Mae Taeng district, Chiang Mai province, northern Thailand, which comprised of 3 species; Melanoides tuberculata, Tarebia granifera, and Thiara scabra. The overall prevalence of pleurolophocercous cercariae was 21.7% as determined by the morphology. For genetic detection of H. taichui infection in snails, 2 primers Hapt_F (5'-GGCCAACGCAATCGTCATCC-3') and Hapt_R (5'-GCGTCGGGTTTCAGACATGG-3'), were used. The genomic DNA of H. taichui, which was used as a positive control, gave an amplification of the 256 bp fragment. The overall prevalence of H. taichui from specific PCR was 9.7%. The proportion of H. taichui among the pleurolophocercous cercariae in this study was 44.9%.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Parasitology
/
Snails
/
Thailand
/
Polymerase Chain Reaction
/
Prevalence
/
DNA Primers
/
DNA, Helminth
/
Heterophyidae
/
Microscopy
Type of study:
Prevalence_studies
/
Risk_factors_studies
Limits:
Animals
Country/Region as subject:
Asia
Language:
En
Journal:
The Korean Journal of Parasitology
Year:
2010
Type:
Article