Changes in Corpus Cavernosum after Partial Bladder Outlet Obstruction in Rat / 대한비뇨기과학회지
Korean Journal of Urology
; : 160-167, 2008.
Article
in Ko
| WPRIM
| ID: wpr-63091
Responsible library:
WPRO
ABSTRACT
PURPOSE: Abnormalities of the relaxation and contraction of the corpus cavernosum can lead to erectile dysfunction. Therefore, we induced a partial bladder outlet obstruction(PBOO) in male rats, and investigated the mechanisms of penile dysfunction with endothelial nitric oxide synthase(eNOS), vascular endothelial growth factor(VEGF), endothelin-1(ET-1), and apoptosis of peri-vascular smooth muscle and connective tissue cells in the corpus cavernosum. MATERIALS AND METHODS: PBOO was induced in 13 Sprague-Dawley rats by placing a 25 gauge needle sheath around the urethra, then ligating the bladder neck with a 3-0 suture. Three week after surgery, distal penile tissues were dissected for immunohistochemical staining, immunoblotting, and TUNEL staining. RESULTS: The expression of eNOS and VEGF were significantly decreased, whereas the expression of ET-1 and apoptosis of perivascular smooth muscle and connective tissue cells were significantly increased in the corpus cavernosum. CONCLUSIONS: The significant increase of ET-1 and apoptosis along with decreased eNOS and VEGF could mediate erectile dysfunction.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Relaxation
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Sutures
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Urethra
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Urinary Bladder
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Urinary Bladder Neck Obstruction
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Immunoblotting
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Rats, Sprague-Dawley
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Apoptosis
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Connective Tissue Cells
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Endothelin-1
Limits:
Animals
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Humans
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Male
Language:
Ko
Journal:
Korean Journal of Urology
Year:
2008
Type:
Article