Pentoxifylline Alleviates Perinatal Hypoxic-Ischemia-Induced Short-term Memory Impairment by Suppressing Apoptosis in the Hippocampus of Rat Pups / 대한배뇨장애요실금학회지
International Neurourology Journal
; : 107-113, 2016.
Article
in En
| WPRIM
| ID: wpr-63261
Responsible library:
WPRO
ABSTRACT
PURPOSE:
Perinatal hypoxic-ischemic brain damage is a major cause of acute mortality and chronic neurologic morbidity in infants and children. We investigated the effects of pentoxifylline, a methylxanthine derivative and type-4 phosphodiesterase inhibitor, on short-term memory and apoptotic neuronal cell death in the hippocampus following perinatal hypoxic-ischemia in newborn rats.METHODS:
We used a step-down avoidance task to evaluate short-term memory and 3ʹ-5ʹ-cyclic adenosine monophosphate (cAMP) assay to detect cAMP levels. We evaluated apoptosis using a terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay for evidence of DNA fragmentation, immunohistochemistry for caspase-3 levels, and western blot for Bcl-2 and Bax.RESULTS:
Perinatal hypoxic-ischemic injury increased apoptotic cell death in the hippocampus, resulting in impaired short-term memory with decreased cAMP levels. Pentoxifylline treatment improved short-term memory by suppressing apoptotic cell death in the hippocampus with elevated cAMP levels.CONCLUSIONS:
Pentoxifylline ameliorated perinatal hypoxic-ischemia in rat pups. This alleviating effect could be ascribed to the inhibition apoptosis due to increased cAMP production by pentoxifylline.Key words
Full text:
1
Index:
WPRIM
Main subject:
Pentoxifylline
/
Brain
/
Immunohistochemistry
/
Adenosine Monophosphate
/
Blotting, Western
/
Mortality
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Cell Death
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Apoptosis
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Cyclic AMP
/
Caspase 3
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
International Neurourology Journal
Year:
2016
Type:
Article