Construction and expression of recombinant plasmid pCD-rbFGF in osteoblasts / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 109-11, 2002.
Article
in En
| WPRIM
| ID: wpr-634038
Responsible library:
WPRO
ABSTRACT
To construct basic fibroblast growth factor (bFGF) eukaryotic expression vector and to evaluate the possibility of bFGF gene therapy in orthopedic disease, the pCD-rbFGF recombinant plasmid was constructed by cloning rat basic fibroblast growth factor (bFGF) cDNA into an eukaryotic expression vector, pcDNA3. Rat osteoblasts were transfected with pCD-rbFGF plasmid by lopofectin mediated gene transfer, the transient expression was detected by streptavidin-biotin-enzyme complex (SABC) method. It was observed that the expression of rat bFGF gene was detected 72 h after transfected distinctly. Basic fibroblast growth factor gene therapy is a method of potential for a wide array of orthopedic diseases.
Full text:
1
Index:
WPRIM
Main subject:
Osteoblasts
/
Plasmids
/
Transformation, Genetic
/
Recombinant Proteins
/
Transfection
/
Cells, Cultured
/
Fibroblast Growth Factor 2
/
Gene Transfer Techniques
/
DNA, Complementary
/
Escherichia coli
Language:
En
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2002
Type:
Article