Phylogenetic Analysis of Orgyia pseudotsugata Single-nucleocapsid Nucleopolyhedrovirus / 中国病毒学
Virologica Sinica
; (4): 257-265, 2007.
Article
in Zh
| WPRIM
| ID: wpr-634185
Responsible library:
WPRO
ABSTRACT
The Douglas-fir tussock moth Orgyia pseudotsugata (Lepidoptera Lymantriidae) is a frequent defoliator of Douglas-fir and true firs in western USA and Canada. A single nucleopolyhedrovirus (SNPV) isolated from O. pseudotsugata larvae in Canada (OpSNPV) was previously analyzed via its polyhedrin gene, but is phylogenetic status was ambiguous. Sequences of four conserved baculovirus genes, polyhedrin, lef-8, pif-2 and dpol, were amplified from OpSNPV DNA in polymerase chain reactions using degenerate primer sets and their sequences were analyzed phylogenetically. The analysis revealed that OpSNPV belongs to group II NPVs and is most closely related to SNPVs that infect O. ericae and O. anartoides, respectively. These results show the need for multiple, concatenated gene phylogenies to classify baculoviruses.
Full text:
1
Index:
WPRIM
Language:
Zh
Journal:
Virologica Sinica
Year:
2007
Type:
Article