Heme oxygenase-1 expression in rats with acute lung rejection and implication / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 84-7, 2009.
Article
in En
| WPRIM
| ID: wpr-635059
Responsible library:
WPRO
ABSTRACT
This study investigated the expression of hemeoxygenase-1 (HO-1) in rats with acute lung rejection and its implication. A valid rat orthotopic left lung transplantation model (SD rat-->Wistar rat) was established by using an improved three-cuff anastomosis technique. The rats were divided into control group, CoPP (HO-1 inducer)-treated group and ZnPP (HO-1 inhibitor)- treated group. The severity of acute rejection was graded on the basis of the morphologic changes of the lung samples stained with HE. The expression of HO-1 protein in lung tissue was detected by using immunohistochemistry and Western blot, and HO-1 mRNA activity was assayed by RT-PCR. The results showed that the expression of HO-1 protein was significantly increased with the acute rejection grading in rats (P0.05). It was concluded that HO-1 protein might be involved in the pathological process of post-graft acute rejection. The expression of HO-1 protein was increased gradually with aggravation of acute rejection, and HO-1 protein might be used as an index to monitor acute rejection after lung transplantation.
Full text:
1
Index:
WPRIM
Main subject:
RNA, Messenger
/
Lung Transplantation
/
Rats, Wistar
/
Rats, Sprague-Dawley
/
Graft Rejection
/
Heme Oxygenase (Decyclizing)
Language:
En
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2009
Type:
Article