A study on ihhibiting effects of MMC on proliferation of fibroblasts derived from human pterygium in vitro / 中华实验眼科杂志
Chinese Journal of Experimental Ophthalmology
; (12): 39-41, 2001.
Article
in Zh
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| ID: wpr-635356
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ABSTRACT
ObjectiveTo evaluate the inhibiting effects of mitomycin C(MMC) on fibroblasts of human pterygium (HPF) in vitro.MethodsThe fibroblasts derived from the 5 th to 8 th generation of the cultured tissues in human pterygium.These fibroblasts were exposed to culture plates with MMC at 6 gradient concentrations,adjusting the concentration value of the culture medium to 105,104,103,102,10 and 1 μg/L.The cells had been treated with PBS in the control group.After 48 h of incubation,the absorbency had been determined individually by MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide] method.The inhibitory rates were calculated accordingly.All the data were analyzed with student t test and linear regression.ResultsThe extensive breakage of fibroblasts was observed.There was a statistical difference between the tested group and control group in absorbency (P<0.05),especially at the concentrations of more than 100 μg/L (P<0.01).The degree of growth inhibition increased with exposure of cells to MMC over 48 h.It showed that there is a positive correlation between the doses of MMC and the antiproliferative effects on HPF.ConclusionMMC significantly inhibites the proliferation of HPF in a dose-dependent manner.
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Language:
Zh
Journal:
Chinese Journal of Experimental Ophthalmology
Year:
2001
Type:
Article