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Expression profiling of MicroRNAs in hippocampus of rats following traumatic brain injury / 华中科技大学学报(医学)(英德文版)
Article in En | WPRIM | ID: wpr-636721
Responsible library: WPRO
ABSTRACT
The changes of microRNA expression in rat hippocampus after traumatic brain injury (TBI) were explored. Adult SD rats received a single controlled cortical impact injury, and the ipsilateral hippocampus was harvested for the subsequent microarray assay at three time points after TBI: 1st day, 3rd day and 5th day, respectively. We characterized the microRNA expression profile in rat hippocampus using the microRNA microarray analysis, and further verified microarray results of miR-142-3p and miR-221 using quantitative real-time PCR. Totally 205 microRNAs were identified and up-/down-regulated more than 1.5 times. There were significant changes in 17 microRNAs at all three time points post-TBI. The quantitative real-time PCR results of miR-142-3p and miR-221 indicated good consistency with the results of the microarray method. MicroRNAs altered at different time points post-TBI. MiR-142-3p and miR-221 may be used as potentially biological markers for TBI assessment in forensic practice.
Full text: 1 Index: WPRIM Language: En Journal: Journal of Huazhong University of Science and Technology (Medical Sciences) Year: 2014 Type: Article
Full text: 1 Index: WPRIM Language: En Journal: Journal of Huazhong University of Science and Technology (Medical Sciences) Year: 2014 Type: Article