Effects of Coix seed oil on human retinal capillary endothelial cells proliferation and VEGF expression in high glucose environment / 国际眼科杂志(Guoji Yanke Zazhi)
International Eye Science
; (12): 2147-2150, 2014.
Article
in Zh
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| ID: wpr-637038
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ABSTRACT
AlM To study the effects of different concentrations of Coix seed oil on human retinal capillary endothelial cells ( HRCECs ) proliferation and vascular endothelial growth factor ( VEGF) expression in high glucose environment. METHODS:
HRCECs extracted from human fresher eyeball and cultured in vitro, and ultimately used in the experiment were the growth of 3rd ~ 4th cells, the experimental were divided into blank control group, low glucose control group, high glucose control group, high glucose + ( 50ü L/mL, 100ü L/mL, 200ü L/mL ) different concentrations Coix seed oil group. Detecting the multiplication of HRCECs by MTT, the immunocytochemical method was employed to detect the each group HRCECs of VEGF expression.RESULTS:
MTT assay results showed that different concentrations of coix seed oil acted at HRCECs for 48h, inhibition of cell proliferation was significant difference compared with high glucose control group (P0. 05). lmmunocytochemical assay showed that50ü L/mL, 100ü L/mL, 200ü L/mL Coix seed oil acted at HRCECs 48h, the expression of VEGF decreased significantly compared with the high glucose control group ( P CONCLUSlONCoix seed oil can inhibit the HRCECs proliferation and suppress the VEGF expression in high glucose environment.
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Language:
Zh
Journal:
International Eye Science
Year:
2014
Type:
Article