Airway Neutrophil Activation and Protease Imbalance in Airway of Children with Foreign-Body Aspiration / 实用儿科临床杂志
Journal of Applied Clinical Pediatrics
; (24)1994.
Article
in Zh
| WPRIM
| ID: wpr-639092
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ABSTRACT
Objective To observe neutrophil activation and protease imbalance in airway of children after foreign-body aspiration(FBA).Methods Bronchoalveolar lavage fluid(BALF) was obtained through fiberoptic bronchoscopy.The supernatant was assayed for the concentration of neutrophil elastase(NE) using enzyme-linked immunosorbent assay(ELISA) and elastase inhibition capacity(EIC)/free elastase activity using a colorimetric assay.The cell pellet were smeared for the expression of ?1-antitrypsin(?1-AT) using cellular immunohisto-chemistry and cytologic analysis by Wright-Giemsa stain.Results Percentage of neutrophil,concentration of NE and the expression of ?1-AT in BLAF group,pair group and pneumonia group significantly increased compared with that of control group(Pa0.05).The ratio of Lib NE in FBA,par and peumcria group was 30.3%,27.3% and 22.7%.Conclusions FBA induces similar airway neutrophil activation and protease imbalance to bacterial pneumonia.The contralateral airway showes the same change as the foreign body side.
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Index:
WPRIM
Language:
Zh
Journal:
Journal of Applied Clinical Pediatrics
Year:
1994
Type:
Article