Analysis of AmpC beta-lactamase gene in Pseudomonas aeruginosa / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 17-9, 23, 2005.
Article
in En
| WPRIM
| ID: wpr-640986
Responsible library:
WPRO
ABSTRACT
The gene and the amino acid sequence of the structural and regulatory region of the Pseudomonas aeruginosa with different resistance patterns were analyzed. Six strains with different resistance patterns were selected and the AmpC beta-lactamase was identified. The objective gene fragment was amplified by colonies PCR. The sequences of the PCR-products were analyzed. The DNA sequence of the structural gene ampC and the regulatory genes ampR, ampD and ampE was detected. The 6 strains and the wild-type Pseudomonas aeruginosa are highly homogeneous in structural and regulatory region. Some new mutant points were found.
Full text:
1
Index:
WPRIM
Main subject:
Pseudomonas aeruginosa
/
Bacterial Proteins
/
Beta-Lactamases
/
DNA, Bacterial
/
Sequence Analysis, DNA
/
Point Mutation
/
Beta-Lactam Resistance
Language:
En
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2005
Type:
Article