Isolation and Culture of Adult Neural Stem Cells from Guinea Pig Tympanic Membrane / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
; : 28-32, 2008.
Article
in Ko
| WPRIM
| ID: wpr-645517
Responsible library:
WPRO
ABSTRACT
BACKGROUND AND OBJECTIVES:
Tympanic membrane perforation is an important clinical problem found in various populations of the world. In large number of cases, acute traumatic perforations heal spontaneously, and in the healing process, stem cells appear to play an important role. However, no studies have been reported regarding somatic stem cells in the tympanic membrane. Herein, we tried to show that guinea pig's tympanic membrane contains cells that display the characteristic features of stem cells. MATERIALS ANDMETHOD:
The tympanic membrane was obtained from the guinea pig. The cells were cultured in a medium with epidermal growth factor (EGF) and fibroblast growth factor (FGF). Proliferating cells were checked with stem cell markers, bromodeoxyuridine (BrdU) and nestin. Differentiated cells from stem cells are checked with betaIII tubulin and S-100.RESULTS:
We observed that some of the cultured cells from the tympanic membrane were stained with both stem cell markers, BrdU and nestin. And we observed that these cells differentiated into neuron and gilal cells, which expressed betaIII tubulin and S-100, respectively.CONCLUSION:
These results suggest that the tympanic membrane of guinea pigs may have neural stem cells. Further study is needed for finding the origin of stem cells.Key words
Full text:
1
Index:
WPRIM
Main subject:
Stem Cells
/
Tubulin
/
Tympanic Membrane
/
Bromodeoxyuridine
/
Cells, Cultured
/
Guinea
/
Tympanic Membrane Perforation
/
Epidermal Growth Factor
/
Adult Stem Cells
/
Neural Stem Cells
Limits:
Animals
/
Humans
Country/Region as subject:
Africa
Language:
Ko
Journal:
Korean Journal of Otolaryngology - Head and Neck Surgery
Year:
2008
Type:
Article