The Relationships between Homocysteine, Folate, MTHER and TSER Polymorphism for Osteoporotic Compression Fracture in Postmenopausal Women / 대한정형외과학회잡지

ABSTRACT

PURPOSE: To analyze the relationships between homocysteine, folate, MTHFR and TSER polymorphism for postmenopausal women with osteoporotic compression fractures. MATERIALS AND METHODS: Forty-three postmenopausal compression fracture patients and as many normal controls were included. The plasma homocysteine and folate levels were measured using a FPIA (fluorescent polarizing immunoassay) kit. The MTHFR and TSER genotypes were amplified by PCR (polymerase chain reaction) and separated by RFLP (restriction fragment length polymorphism) in a 3.5% agarose gel. RESULTS: The plasma folate level was significantly lower in the postmenopausal women with osteoporotic compression fractures, particularly in the MTHFR 677CT and TSER 2R (-) genotypes. However, the plasma homocysteine level and MTHFR C677T polymorphism were similar to the control group. CONCLUSION: A low folate level and the TSER 2R (-) genotype can be associated with osteoporotic compression fractures in postmenopausal women.