Porous Microcarrier-Enabled Three-Dimensional Culture of Chondrocytes for Cartilage Engineering: A Feasibility Study
Tissue Engineering and Regenerative Medicine
; (6): 235-241, 2016.
Article
in En
| WPRIM
| ID: wpr-649703
Responsible library:
WPRO
ABSTRACT
Cartilage repair is substantially intractable due to poor self-healing ability. Porous microspheres can be a fascinating three-dimensional matrix for cell culture and injectable carrier in cartilage engineering. In this study, we assessed the feasible use of porous biopolymer microspheres for chondrocyte carriers. When seeded onto the blended biopolymer microspheres and followed by a dynamic spinner flask culture, the chondrocytes showed robust growth behaviors during the culture period. The gene expressions of SOX9, type II collagen, and aggrecan were significantly upregulated after 2-week of culture. Furthermore, immunolocalization of type II collagen and secretion of glycosaminolglycan became prominent. The results suggest the feasible usefulness of the porous microspheres as the cell culture matrix and the subsequent delivery into cartilage defects.
Key words
Full text:
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Index:
WPRIM
Main subject:
Biopolymers
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Cartilage
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Gene Expression
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Feasibility Studies
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Cell Culture Techniques
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Chondrocytes
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Collagen Type II
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Aggrecans
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Microspheres
Language:
En
Journal:
Tissue Engineering and Regenerative Medicine
Year:
2016
Type:
Article