The Effect of IP3 Receptor Inhibition Using 2-APB on Gentamicin Ototoxicity in Cochlear Sensory Cell / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
;
: 385-390, 2007.
Article
in Korean
| WPRIM
| ID: wpr-650022
ABSTRACT
BACKGROUND AND OBJECTIVES:
Elevated intracellular calcium level is known to play important roles in the apoptotic pathway. IP3 receptor (ligand-gated channels that release Ca2+ from intracellular stores) is emerging as a key site for regulation of apoptosis. 2-Aminoethoxydiphenyl borate (2-APB) is one of the reliable IP3 receptor antagonists. We examined the effect of 2-APB on gentamicin ototoxicity in vitro, using the HEI-OC1 cell line. MATERIALS ANDMETHOD:
HEI-OC1 cells were trWWeated with 100micrometer gentamicin. Using a CaspACE assay, we measured the caspases-3 activity in the gentamicin treated hair cells with and without 2-APB pre-incubation. We also observed intra-cellular calcium concentrations in HEI-OC1 cells using a confocal microscopy (calcium green-1 stain). Live cell imaging was performed by using fluorescence video-time lapse system.RESULTS:
Cytosolic calcium elevation by gentamicin was remarkably inhibited by 2-APB. Caspases-3 activities of gentamicin treated cells were higher than those of the control. After incubation with 2-APB, caspases-3 activities and cell death of gentamicin treated cells were shown to decrease.CONCLUSION:
2-APB reduces Caspases-3 activity in the gentamicin treated HEI-OC1 cells by inhibition of cytosolic calcium increase.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Gentamicins
/
Cell Line
/
Calcium
/
Cell Death
/
Apoptosis
/
Microscopy, Confocal
/
Cytosol
/
Caspase 3
/
Inositol 1,4,5-Trisphosphate Receptors
/
Fluorescence
Language:
Korean
Journal:
Korean Journal of Otolaryngology - Head and Neck Surgery
Year:
2007
Type:
Article
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