Sample type is vital for diagnosing infection with peste des petits ruminants virus by reverse transcription PCR

Pam-Dachung LUKA; Chrisostom AYEBAZIBWE; David SHAMAKI; Frank-Norbert MWIINE; Joseph ERUME

Pam-Dachung LUKA; Chrisostom AYEBAZIBWE; David SHAMAKI; Frank-Norbert MWIINE; Joseph ERUME.

J. vet. sci ; J. vet. sci;: 323-325, 2012.

Article in En | WPRIM | ID: wpr-65159

Responsible library: WPRO

ABSTRACT

ABSTRACT

Peste des petits ruminants (PPR) diagnosis from suspected samples from sheep and goats was carried out. Buffy coat, tissues, and oculo-nasal swabs were analyzed using nucleoprotein (NP3/NP4) and fusion protein (F1/F2) gene primers, respectively. Analysis of the sample types and primer set revealed that buffy coat are the best type of samples for PPR diagnosis and the use of two set of primers will increase the number of positives.

Subject(s)

Animals; DNA Primers/analysis; Eye/virology; Goat Diseases/blood; Goats; Hair/virology; Nose/virology; Nucleoproteins/analysis; Peste-des-Petits-Ruminants/blood; Peste-des-petits-ruminants virus/genetics; Pigmentation; RNA, Viral/genetics; Reverse Transcriptase Polymerase Chain Reaction/methods; Sheep; Sheep Diseases/blood; Uganda/epidemiology

Key words

diagnosis; goats; peste des petits ruminants; sample; sheep

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Full text: 1 Index: WPRIM Main subject: Sheep Diseases / Uganda / Pigmentation / RNA, Viral / Goats / Sheep / Goat Diseases / Nose / Peste-des-petits-ruminants virus / DNA Primers Type of study: Diagnostic_studies Limits: Animals Country/Region as subject: Africa Language: En Journal: J. vet. sci Year: 2012 Type: Article

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