Evidence for estrogen receptor expression during medullary bone formation and resorption in estrogen-treated male Japanese quails (Coturnix coturnix japonica)
Journal of Veterinary Science
;
: 223-227, 2012.
Article
in English
| WPRIM
| ID: wpr-65172
ABSTRACT
The temporal expression of estrogen receptor (ER)-alpha and ER-beta mRNA was examined in male Japanese quails. Femurs of quails receiving 17beta-estradiol underwent RTPCR and histochemical analysis 1 to 15 days after treatment. Untreated quails were used as controls (day 0). Between days 0 and 5, cells lining the bone endosteal surface differentiated into osteoblasts, which in turn formed medullary bone. Expression of ER-alpha was already observed on day 0 and increased slightly during bone formation whereas ER-beta was hardly detected throughout this process. After osteoclasts appeared on the medullary bone surface, this type of bone disappeared from the bone marrow cavity (days 7~15). ER-alpha expression simultaneously decreased slightly and ER-beta levels remained very low. These results suggest that estrogen activity mediated by ER-alpha not only affects medullary bone formation but also bone resorption.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
Osteogenesis
/
Bone and Bones
/
Bone Resorption
/
RNA, Messenger
/
Cells, Cultured
/
Gene Expression Regulation
/
Coturnix
/
Reverse Transcriptase Polymerase Chain Reaction
/
Estrogen Receptor alpha
Limits:
Animals
Language:
English
Journal:
Journal of Veterinary Science
Year:
2012
Type:
Article
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