Isolation of Voltage Dependent Calcium Current in Chick Inner Hair Cell / 대한이비인후과학회지
Korean Journal of Otolaryngology - Head and Neck Surgery
; : 655-659, 2009.
Article
in Ko
| WPRIM
| ID: wpr-652152
Responsible library:
WPRO
ABSTRACT
BACKGROUND AND OBJECTIVES: Voltage dependent calcium channel (VDCC) mediates calcium ion influx and controls neurotransmitter release in excitable cells. Hair cells in vertebrates cochlea are known to express L-type VDCC. The purpose of this study was to measure calcium current from hair cells to investigate basic activity and characteristics of VDCC. MATERIALS AND METHOD: We measured calcium current in hair cells of the chicken's auditory organ, the basilar papilla analogous to the mammalian cochlea, in whose L-type, dihydropyridinesensitive calcium channels predominate and in vestibular hair cells from cristae. Calcium currentthrough VDCC was isolated in voltage-clamp recording using Cesium, Tetraethylammonium, 4- aminopyridine and apamin to block the much larger potassium currents. Various concentrations of internal calcium buffer, ethylene glycol tetraacetic acid (EGTA) or 1,2-bis (o-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid (BAPTA) were used. RESULTS: The higher the buffer concentration, the larger the current size were ; they were significantly larger in 10 mM of calcium buffer concentration (ANOVA, p< 0.05). There was no difference in calcium current between cochlear and vestibular hair cells. CONCLUSION: We could successfully isolate stable inward calcium current from chick hair cells. This experiment can be used as a basic method to understand neurotransmission process between hair cells and afferent neurons.
Key words
Full text:
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Index:
WPRIM
Main subject:
Organ of Corti
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Apamin
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Potassium
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Vertebrates
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Calcium Channels
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Cesium
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Calcium
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Egtazic Acid
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Cochlea
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Hair Cells, Vestibular
Language:
Ko
Journal:
Korean Journal of Otolaryngology - Head and Neck Surgery
Year:
2009
Type:
Article