Agonistforthe Control of Apotosis through the Study of Cytokine Expression after Spinal CordInjuryin Rats / 대한정형외과학회잡지
The Journal of the Korean Orthopaedic Association
; : 106-114, 2007.
Article
in Ko
| WPRIM
| ID: wpr-654458
Responsible library:
WPRO
ABSTRACT
Purpose: To analyze the cytokines that appear after a spinal cord injury in rats and to determine the agonists that regulate apoptosis. Materials and Methods: Sixty female Sprague-Dawley rats were anesthetized, and a laminectomy was performed at the 9th thoracic vertebra. The spinal cord injury was induced by dropping a 10 gm weight at a height of 20 mm. The subjects were divided into 5 groups. Group I was administered aminoguanidine, group II was administered GM-CSF, group III was administered riluzole, group IV was administered erythropoietin, and group V was administered methylprednisolone. A control group was administered normal saline. The results were assessed using the Tarlov motor grading method. In 1, 3, 5 and 7 days after the spinal cord injury, the rats were sacrificed and evaluated using the syringomyelic cavity size. Immunohistochemical staining for e-NOS and RT-PCR for XIAP were also performed. Results: Groups I, III, and V showed significantly different results in terms of the motor recovery and inhibition of increase in the syringomyelic cavity compared with the other groups (p<0.05). The e-NOS activity was observed in groups I, III, and V. The other groups showed almost no e-NOS activity. On the RT-PCR, groups I, III, and V showed significantly different results in terms of XIAP expression with time compared with the other groups. Conclusion: Steroids, NOS inhibitors and sodium channel inhibitors appear to be important factors for regulating apoptosis in the early stage of a spinal cord injury. Further study will be needed to develop new pharmaceuticals with synergic effects on spinal cord injuries.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Spinal Cord Injuries
/
Spine
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Steroids
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Methylprednisolone
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Cytokines
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Granulocyte-Macrophage Colony-Stimulating Factor
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Erythropoietin
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Rats, Sprague-Dawley
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Apoptosis
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Riluzole
Limits:
Animals
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Female
/
Humans
Language:
Ko
Journal:
The Journal of the Korean Orthopaedic Association
Year:
2007
Type:
Article