Effect and Mechanism of Tea Polyphenols on Morphine-induced Constipation / 医药导报

ABSTRACT

Objective To explore the preventive effect and mechanism of tea polyphenols on morphine-induced constipation. Methods Female Kunming mice were randomly divided into 4 groups ( 10 mice per group) , including blank control group, model control group, tea polyphenols group and tea polyphenols+morphine group. Tea polyphenols group and tea polyphenols + morphine group were pretreated with 100 mg·kg-1 of tea polyphenols for 4 days, meanwhile blank control group and model control group were preteated with 0.1 mL·kg-1 of 0.5% CMC-Na for 4 days. On the fourth day model control group and tea polyphenols + morphine group were intraperitoneal injected 20 mg kg-1 morphine, otherwise blank control group and tea polyphenols group were injected with 0.1 mL·kg-1 of 0.9% sodium chloride solution. Then mice were given 0.2 mL of 5% inksolution by intragastric administration 15 min later. The latency to paw licking was detected in hot plate test to evaluate the effect of tea polyphenols on morphine analgesia. The levele of motilin (MLT), substance P (SP) and somatostatin (SS) in intestinal tissue were determined by enzyme-linked immunosorbent assay ( ELISA) among groups. Results Compared with the blank control group, the length of propelling ink and the propelling rate of ink were significantly lower in the model control group ( P<0.01) , meanwhile tea polyphenols group were much higher ( P<0. 05 ) . Compared with model control group, the length of propelling ink and the propelling rate of ink were significantly higher in tea polyphenols + morphine group mice ( P<0.05) . The first paw licking time of control group and tea polyphenols group were (8.64 + 2.72)s and (9.11 + 2.13) s, and the time of model control group and tea polyphenols + morphine group were (18.79±3.58)s and (20.10±3.72) s. The contents of MLT and SP were reduced in model control group (P<0.05), but significantly increased in tea polyphenols group (P<0.05) compared with blank control group. Compared with the model control group, MLT and SP had an obviously increase in tea polyphenols +morphine group (P<0.05). Compared with blank control group, the content of SS was increased in model control group, but decreased markedly in tea polyphenols group and tea polyphenols+morphine group ( P<0.05) . Conclusion Tea polyphenols can prevent the morphine-induced constipation without decreasing the analgesic effect of morphine, which is related to the regulation of the content of MLT, SP and SS.