Study on expression and change of CRLR, CGRP-RCP in venous blood of New Zealand rabbit with allergic rhinitis / 重庆医学

ABSTRACT

Objective To observe the expressions and change of calcitonin receptor-like receptor(CRLR) and calcitonin generelated peptide receptor component protein(CGRP-RCP)in venous blood of New Zealand rabbit with allergic rhinitis and to analyze its effective mechanism.Methods Twenty-four New Zealand rabbits were divided into the normal control group(A) and allergic rhinitis group(B).Ovalbumin was used to immunize New Zealand rabbits and conduct the nasal cavity stimulation for preparing allergic rhinitis model.The rabbit behavior change was observed in the two groups.The turbinate and nasal septum mucosa were taken for preparing HE sections.The pathologic change of nasal mucosa was observed.The venous blood was collected for detecting CRLR and CGRP-RCP levels change by ELISA.Results The behavior scores showed that the behavior score on 1-5 d after nasal cavity stimulation had statistical difference between the two groups(P＜0.05).The HE staining displayed that the nasal cavity mucosa and mesenchyma had slightly edema with blood vessels mild hyperplasia and congestion,the inflammatory cells infiltration was unobvious,and had little nasal secretions.The group B had obvious interstitial edema,vascular hyperplasia,congestion and a large number of eosinophils and other inflammatory cells infiltration.The CRLR and CGRP-RCP levels in the group B were significantly increased compared with the group A(t=5.143,10.595,P＜0.05).Conclusion CRLR and CGRP-RCP may play an important effect in pathogenesis of allergic rhinitis.