Effect of microRNA-7 knockdown on ConA-induced acute liver injury in mice / 中国病理生理杂志
Chinese Journal of Pathophysiology
;
(12): 1643-1647, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-662742
ABSTRACT
AIM:
To study the effect of microRNA-7 (miR-7) knockdown (KD) on concanavalin A (ConA)-induced acute liver injury (ALI) in mice.METHODS:
Wild type (WT) mice and miR-7KD mice were received ConA (30 mg/kg) to induced acute liver injury model by intraperitoneal injection,and the morphological changes,liver weight and weight index were measured 48 h later.The pathological changes of the liver tissues were observed by HE staining.The levels of serum alanine aminotransferase (ALT),IL-4 and IFN-γ were detected by ELISA.The proportional changes of CD4 + T cells and the relative levels of IL-4 and IFN-γwere analyzed by flow cytometry.RESULTS:
The color of the liver tissue became lighter,and the weight and weight index were changed significantly in miR-7KD mice compared with control group (P < 0.05).HE staining showed that the inflammatory cell infiltration was increased in the liver of miR-7KD mice.Moreover,the level of serum ALT was significantly increased (P < 0.05).The serum level of IFN-γelevated significantly (P < 0.01),while the IL-4 levels decreased significantly (P < 0.01) in the serum of miR-7KD mice.Furthermore,the proportion of CD4 + T cells and relative IFN-γcells increased obviously (P < 0.01).CONCLUSION:
miR-7 knockdown promotes the pathogenesis of the ConA-induced acute liver injury in mice.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
2017
Type:
Article
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