Study on HPLC Fingerprint of Stir-baked Manis pentadactyla / 中国药房
China Pharmacy
;
(12): 3839-3842, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-662944
ABSTRACT
OBJECTIVE:
To establish HPLC fingerprint of stir-baked Manis pentadactyla.METHODS:
HPLC method was conducted.The determination was performed on Capcell Pak Mg Ⅱ S5 C18 column with mobile phase consisted of acetonitrile-0.1% phosphoric acid solution (gradient elution) at the flow rate of 0.8 mL/min.The detection wavelength was set at 275 nm,and column temperature was 30 ℃.The sample size was 10 μL.Using oxyphenylaminopropionic acid as reference,HPLC chromatograms of 11 batches of medicinal materials were determined.TCM Chromatogram Fingerprint Similarity Evaluation System (2004 A edition) was used for common peak identification and similarity evaluation.RESULTS:
There were 23 common peaks in 11 batches of stir-baked M.pentadactyla,with similarity>0.90.After validation,HPLC chromatograms of 11 batches of medicinal materials were in good agreement with control fingerprints.CONCLUSIONS:
Established HPLC fingerprint can provide reference for the identification and quality evaluation of stir-baked M.pentadactyla.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2017
Type:
Article
Similar
MEDLINE
...
LILACS
LIS