Preparation of Silicon and Boron-doped Carbon Dots and Their Application in Hemoglobin Sensing / 分析化学

ABSTRACT

Heteroatom doping is an effective way to elevate the fluorescent performance of carbon dots. In this study, a microwave one-pot approach for the synthesis of SiBCDs was proposed by using citric acid ( C6 H8 O7 ) , boric acid ( H3 BO3 ) and (3-aminopropyl) triethoxy sliane ( APTES) as source materials. PAAS-SiBCDs were prepared with the assistance of microwave when sodium polyacrylate ( PAAS) was added in the precursor of SiBCDs. The products were characterized by X-ray diffraction ( XRD ) , X-ray photoelectron spectroscopy ( XPS ) and Fourier transform infrared ( FT-IR ) spectra, respectively, confirming that the product was amorphous CDs, with small amount of Si and B. The synthesized SiBCDs had good monodispersity with size of 4-8 nm, the average size of PAAS-SiBCDs was 5. 2 nm, and the excitation/emission maximum was 350 nm/445 nm. The quantum yield of SiBCDs was 20. 1%, and that of PAAS-SiBCDs was 34. 6%. Based on the quenching effect of hemoglobin ( Hb ) on the fluorescence PAAS-SiBCDs, a sensitive Hb detection method was developed. A linear range of 0. 21-5. 22 μmol/L was obtained together with a detection limit of 0. 06 μmol/L. The method was applied in the determination of Hb in human blood samples.