The mechanism of IL-17A induced the secretion of CXCL13 in gastric cancer cell line SGC-7901 / 国际检验医学杂志

ABSTRACT

Objective To study the molecular mechanism of interleukin-17A(IL-17A)induced the secretion of CXCL13 in gas-tric cancer cell line SGC-7901.Methods SGC-7901 cells were stimulated by recombination cytokine IL-17A,or SGC-7901 were pre-incubated with signal pathway inhibitor for 1 hour and then stimulated by IL-17A,the level of CXCL13 were detected by using en-zyme-linked immunosorbent assay.Results After IL-17A stimulation,the secretion of CXCL13 by SGC-7901 cells was significant-ly increased(P<0.05),which was with dose and time dependence.IL-17A-induced secretion of CXCL13 could be inhibited when SGC-7901 were pre-incubated with STAT3 inhibitor(P<0.05).However,such effect was not observed while SGC-7901 were pre-incubated with NK-κB,MEK1/2,p38/MAPK and JNK inhibitors.Conclusion IL-17A could induce the secretion of CXCL13 in gastric cancer cell line SGC-7901 by activating STAT3 signal pathway,which might play regulation role in gastric cancer.