Effect of simulated transportation vibration on suspended erythrocyte quality / 军事医学

ABSTRACT

Objective To investigate the effect of vibration on the quality of suspended erythrocytes during transportation, and explore suitable experimental vibration conditions.Methods Three intensities(highway truck vibration, random vibration of fixed goods in a caterpillar, and combined wheeled vehicle vibration)were selected to simulate suspended erythrocyte transportation using electromagnetic vibration test system.Suspended erythrocytes of the same storage were randomly divided into three groups:highway truck vibration(group1),random vibration of fixed goods in a caterpillar(group 2),and combined wheeled vehicle vibration(group 3).The suspended erythrocytes were stored for 11 days and 26 days.The control group was stored with conventional methods.Suspended erythrocytes were vibrated for 1 hour,samples were collected before and after vibration,while free hemoglobin(FHb),K+,and LDH were tested.Results The changes in FHb and LDH after vibration were gradually increased with the magnitude of vibration(P<0.05).There was no significant difference in K +between the three vibration levels.The increase in FHb in suspended erythrocytes stored for 26 days was higher than 11days after random vibration of fixed goods in a caterpillar and combined wheeled vehicle vibration(P<0.05).There was no significant difference in the change in FHb between day(d)26 and d 11 after highway truck vibration.Under the same magnitude of vibration,the change in LDH in the d 26 suspended erythrocytes was more significant than that of the d 11, and no significant difference was found in the change in K +between d 26 and d 11. Conclusion The damage to suspended erythrocytes after combined wheeled vehicle vibration is more obvious than that by the other two vibrations.The vibration environment of combined wheel vehicles is more suitable for simulating the vibration damage to suspended erythrocyte vibration.Suspended erythrocytes with a longer storage time have significant changes in FHb and LDH after transportation vibration, and the length of storage time of suspended red blood cells might affect the vibration injury.