Effect of Niaogan Decoction on expulsion of uropathogenic Escheriehia coli from human bladder epithelial cells / 中成药

ABSTRACT

AIM To observe the effects of Niaogan Decoction (Portulacae Herba,Taraxaci Herba) on expulsion of uropathogenic Escheriehia coli (UPEC) from human bladder epithelial cells and to explore its mechanism.METHODS After HTB-9 was infected with UPEC,drug urine exocytosed the UPEC invaded into urinary bladder epithelial cell were observed.With molecular biology technology,the mechanism of key factors to TLR4/cAMP signal pathway such as Toll-like receptor 4 (TLR4),adeuylate cyclase 3 (AC3),cyclic adenosine monophosphate (cAMP),protien kinase A (PKA),Myosin Ⅶ A and Rab interacting protein (MyRIP),Rab27b and Caveolin-1 were analyzed.RESULTS Treatment with Niaogan Decoction resulted in a significant increase in UPEC exocytosis compared with the blank control high dose group.Niaogan Decoction group improved TLR4,AC3,MyRIP,Rab27b and Caveolin-1 protein expression,increased intracellular cAMP content and promoted PKA activation.CONCLUSION Niaogan Decoction has the capacity to exocytose UPEC,its mechanism may relate to TLR 4/cAMP signal pathway.