Treatment of coronary micro-circulation thrombus by dodecafluoropentane acoustic vaporizable nano-droplet with ultrasound irradiation:an in vitro experimental study / 中华超声影像学杂志

ABSTRACT

Objective To explore the clinical value of a new tactic with novel dodecafluoropentane (C5F12,DDFP)acoustic vaporizable nano-droplets for the therapy of coronary micro-circulation thrombolysis.Methods Group A was poly butylene succinate buffer solution as blank control;group B was SonoVue microbubbles;group C was DDFP acoustic vaporizable nano-droplets,which were produced with emulsion process by ultrasonic oscillation instrument and rotary evaporator.The size of the nano-droplet was tested by Malvern laser particle size detector and observed with fluorescence microscope.The stability and efficiency of cavitation effect of group C for thrombolysis were tested in an in-vitro artificial vascular system with arterial thrombus model,comparing with group A and B.Weights and pathological sections of the inserted thrombus before and after the thrombolysis in these groups were compared for the thrombolytic efficiency.Results The average size of DDFP nano-droplets were(424.7±30.2)nm.More than 75% of the nano-droplets were ranged from 300-750 nm.The fluorescence microscope showed the DDFP nano-droplets were uniformed round-shaped and stable-structured with favorable dispersity;the nano-droplets had gradually turned into over 3 μm microbubbles under ultrasound irradiation.The thrombus weight loss after thrombolysis in group C[(199.0±35.8)mg,(32.1 ±4.4)%]was significantly larger than those in group A[(30.2 ± 17.8)mg,(5.0 ± 2.4)%]and B[(72.6 ± 20.7)mg,(12.7 ± 2.8)%](all P <0.01). Conclusions DDFP vaporizable nano-droplets with ultrasound irradiation is effective for thrombus elimination in coronary micro-circulation through lasting and stable cavitation effect.