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Knockdown of growth hormone receptor prevents growth hormone in-duced inflammatory cytokine production in 3T3-L1 adipocytes / 中国病理生理杂志
Chinese Journal of Pathophysiology ; (12): 2090-2094, 2017.
Article in Zh | WPRIM | ID: wpr-667645
Responsible library: WPRO
ABSTRACT
AIM:To investigate the effect of growth hormone receptor(GHR) knockdown on nuclear factor-κB (NF-κB) activity and inflammatory cytokine production stimulated by growth hormone (GH) in 3T3-L1 adipocytes. METHODS:The specific siRNA for GHR was transfected into 3T3-L1 adipocytes to silence GHR expressions. The effects of GH on NF-κB activation and inflammatory cytokine production in 3T3-L1 adipocytes transfected with siRNA-GHR or siRNA-control were measured by dual-luciferase system analysis,real-time RT-PCR and ELISA. RESULTS:The protein expression of GHR was diminished after transfection with GHR specific siRNA. Dual-luciferase reporter system analysis re-vealed that GHR knockdown resulted in attenuation of GH-stimulated NF-κB activation in the 3T3-L1 adipocytes. GHR knockdown ameliorated the GH-induced production of inflammatory cytokines TNF-α,IL-1β,IL-6,MCP-1 and MIP-1α in the 3T3-L1 adipocytes. CONCLUSION:Knockdown of GHR might be efficacious to prevent GH-induced inflammatory re-sponses in the 3T3-L1 adipocytes.
Key words
Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Journal of Pathophysiology Year: 2017 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Journal of Pathophysiology Year: 2017 Type: Article