Determination of Notoginsenoside R1,Ginsenoside Rg1 and Ginsenoside Rb1 in Compound Huanggen Gran-ules by HPLC / 中国药师
China Pharmacist
; (12): 55-57,58, 2015.
Article
in Zh
| WPRIM
| ID: wpr-671104
Responsible library:
WPRO
ABSTRACT
Objective:
To set up a method for simultaneously determining the content of notoginsenoside R1 , ginsenoside Rg1 and ginsenoside Rb1 in compound Huanggen granules ( sugar free) .Methods:
The HPLC method was carried out on an Agilent ZORBAX SB-C18(250 mm ×4.6 mm,5 μm)column. The mobile phase was acetonitrile-water with gradient elution. The flow rate was 1.0 ml· min-1 . The detection wavelength was at 203 nm. The column temperature was set at 25℃ and the sample size was 10 μl.Results:
The linearity of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 was within the range of 1. 6-10. 0 μg·ml-1(r=0. 999 6), 6. 3-39. 3 μg·ml-1(r=0. 999 8) and 6. 3-39. 7 μg·ml-1(r=0. 999 7), respectively. The average recovery was 98. 81%(RSD=1. 20%),99. 93%(RSD=0. 93%) and 99. 22%(RSD=0. 87%)(n=6) , respectively.Conclusion:
The method is simple, repro-ducible and specific, which can be used in the quality control of the preparation.
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Index:
WPRIM
Language:
Zh
Journal:
China Pharmacist
Year:
2015
Type:
Article