Transplantation of schwann’s cells transfected by brain derived neurotrophin factor gene into electrical reticular lesion of the brain stem in rats / 中华创伤杂志

ABSTRACT

Objective To investigate the effect of brain derived neurotrophin factor (BDNF) transfected with cationic liposome in protecting nerve tissue and promoting regeneration of the myelin sheath. Methods Wistar rat Schwanns cells (SWCs) were cultured in vitro. The recombinant cationic liposomes mediated PCDNA 3 BDNF was transplanted into SWCs and then into electrical reticular lesion of the brain stem (Group Ⅰ). At the same time, pure cell transplantation group (Group Ⅱ), empty transfected vector cell transplantation (Group Ⅲ) and normal saline injection group (Group Ⅳ) were set. The concentration of BDNF in the brain stem was measured by ELISA, the survival of myelinated basic protein (MBP) and SWCs in the brain stem by immunohistochemistry and the regeneration of the myelin sheath by the transmission electron microscope (TEM). Results One week after transplantation, the concentration of BDNF in the brain stem in the Group Ⅰ was much higher than that in other three groups ( P