The effect of angiotensin Ⅱon cardiac hypertrophy in SD rat with different ages / 中华老年医学杂志

ABSTRACT

Objective The effect of angiotensinⅡ(AngⅡ) on cardiac hypertrophy were studied in SD rat with different ages. Methods Cardiocyte and cardiac fibroblasts in neonatal SD rat group and 12 months SD rat group were cultured in vitro. In each ages group, control MEM, angⅡ, angⅡantagonist(Losartan) and angⅡ+losartan were administrated respectively. 3 H leu incorporation into cardiocyte and 3 H TdR incorporation into cardiac fibroblasts were measured in neonatal rat group and 12 month rat group. To investigate the effect of cardiac fibroblasts on cardiocyte under the influence of angiotensinⅡ, the supernatant of cultured cardiac fibroblasts with angiotensinⅡ, the supernatant of cultured CBS with losartan and the supernatant of cultured cardiac fibroblasts with angiotensinⅡ and Losartan were added into cultured cardiocyte respectively. 3 H leu incorporation into cardiocyte were tested. Results 10 6 mol/L angⅡcan evidently augment 3 H leu incorporation into cardiocyte ( P