Effect of Ischemia-Reperfusion Injury on Apoptosis of Pancreatic Cells in Rats with Acute Pancreatitis / 中国普外基础与临床杂志
Article
in Zh
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| ID: wpr-675818
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ABSTRACT
Objective To investigate the effect of ischemia reperfusion (I/R) injury on apoptosis of pancreatic cells in rats with acute pancreatitis(AP). Methods Fifty four SD rats were randomized into 3 groups: pancreatitis group ( n =24), I/R injury group ( n =24) and control group ( n =6). The animal model of AP was induced by retrograde injection of 3% sodium taurocholate into biliopancreatic duct in rats. Pancreatic I/R was caused by blocking the inferior splenic artery and removing the clamp after AP induction. At 1 h, 3 h, 6 h and 12 h, groups of rats were sacrificed. A terminal deoxynucleotidyl transferase mediated dUTP biotion nick end labeling (TUNEL) was used to detect pancreatic apoptosis, and histological changes of the pancreas were observed. Results Pancreatic hemorrhage, necrosis were respectively observed in the pancreatitis rats at 6 h and the I/R injury rats at 1 h. Histological changes of the pancreatitis rats at 1 h and 3 h were only congestion and edema. Apoptoic acinar cells increased after AP induction, the peak respectively appeared at 6 h in the pancreatitis rats and at 3 h in the I/R injury rats. Compared with the pancreatitis rats, apoptosis index (AI) of the I/R injury rats was significantly higher at 1 h and 3 h ( P
Full text:
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Index:
WPRIM
Type of study:
Clinical_trials
Language:
Zh
Journal:
Chinese Journal of Bases and Clinics in General Surgery
Year:
2003
Type:
Article