The relationship between hepatitis B virus pre-S gene mutations and progression of liver disease / 中华传染病杂志
Chinese Journal of Infectious Diseases
; (12)2001.
Article
in Zh
| WPRIM
| ID: wpr-679835
Responsible library:
WPRO
ABSTRACT
Objective To study the relationship between hepatitis B virus(HBV)pre-S gene mutations and progression of liver disease.Methods The entire pre-S1,pre-S2 genes were amplified hy nested polymerase chain reaction(PCR)and the products were digested by NlaⅢ.The method for detecting pre-S2 start codon mutation was established based on the digested restriction fragment length polymorphism(RFLP).Pre-S gene deletion was revealed by electrophoresis on polyacrylamide gel(PAGE).Pre-C A1896 and basic core promoter T1762/A1764 mutations were identified by direct sequencing of PCR products.The 138 sera from patients with HBV-related disease,including asymp- tomatic carriers(ASC),chronic hepatitis(CH),liver cirrhosis(LC),hepatocarcinoma(HCC),were tested by these methods.Results The detection rate of pre-S deletion mutation was higher in patients with HCC(56.3%)and LC(42.9%)than those with CH(11.8%)and ASC(8.1%,P
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Infectious Diseases
Year:
2001
Type:
Article