Preliminary attempt to identify geoherbalism of Flos Lonicerae by sequence divergence of 5S-rRNA gene spacer region / 中草药

ABSTRACT

Object To study the formation of geoherbalism of Flos Lonicerae on the molecular level Methods Genomic DNAs from different populations of Lonicera japonica Thunb , outgroup Lonicera similis Hemsl , and Lonicera confusa DC were extracted, the 5S rRNA gene spacer region amplified, sequuenced, and analysed with Mega 1 02 Results The fragments of 5S rRNA gene spacer region in Lonicera L was about 210 bp with G+C content up to about 70% The sequences were different in various populations, and can be identified by sequencing The genetic distance between L confusa and L japonica was larger Conclusion The genuine distance between species was larger than that within species; the genetic distance among genuine crude drugs was smaller; the genetic distance among genuine and ungenuine crude drugs were bigger than that among genuine crude drugs