Establishment of asexual multiplication system of Arnebia euchroma / 中草药

ABSTRACT

Object In order to promote the artificial cultivation of Arnebia euchroma (Royle) Johnst , and save the wild herb from relieving the lack of resources, the asexual multiplication system of A euchroma was established by tissue culture technique Methods Dedifferentiation in many kinds of explants of A euchroma by inducement succeeded After the embryos were multiplied, there were a lot of germinated transplants in media After callus were multiplied, many adventitious buds had formed in the media, then they were rooting; the transplants formed by inducement and the plants survived after being cultured in soil Results During the dedifferentiation, 2,4 D was essential, the effect of KT was better than BA The shoot sections were dedifferentiated in the most rapid way among the explants The callus from them were in very good quality It is of benefit to the callus multiplication and redifferentiation after reducing the concentration of 2,4 D Both KT and BA can induce adventitious buds, KT was also suitable for the formation of globular shaped embryos In initial period of sphere embryos culture, it was necessary to add the plant growth regulators in media for multiplying sphere embryos and promoting their development Proper temperature difference between day and night (10 ℃-28 ℃) and natural light were helpful for the formation of strong sprouts and survival of transplants The survival rate was 13 2% with 31 plantlets survived among 236 transplants cultured in soil Conclusion The asexual multiplication system of A euchroma established through tissue culture is stable and available