Determination of Tanshinone Ⅱ_A and Schizandrol A in Anshenbuxin Pill by HPLC / 中成药

ABSTRACT

AIM: To develop a method to determine the content of Tanshinone Ⅱ A and Schizandrol A in Anshenbuxin Pill(Radix Salviae Miltiorrhizae, Fructus Schisandrae Chinensis, Rhizoma Acori Tatarinowii,etc.). METHODS: HPLC was used to determine the content of Tanshinone Ⅱ A and Schizandrol A in Anshenbuxin Pill. The separation was performed on C 18 column with methanol water (80∶20) for Tanshinone Ⅱ A and methanol water(50∶50) for Schizandrol A, respectively. The detection wavelength was at 270nm for Tanshinone Ⅱ A and 250nm for Schizandrol A, respectively. RESULTS: The average recovery of Tanshinone Ⅱ A was 98.54%( RSD =1.75%, n =6) and the average recovery of Schizandrol A was 99.05%( RSD =1.77%, n =6). CONCLUSION: This method is simple, accurate with good reproducibility and can be used to control the quality of Anshenbuxin Pill.