Experimental studies of implantation of autologous murine endothelial progenitor cells(EPC)to promote neovascularization in ischemic myocardium / 中华急诊医学杂志

ABSTRACT

Objective To explore the procedures of isolation,cultivation of endothelial progenitor cells(EPC) from peripheral bloods and the feasibility of local EPC transplantation to promote neovascularization in ischemic myocardium. Methods Peripheral blood was obtained from the femoral artery of each Sprague-Dawley(SD)rat.Myocardial ischemia was induced by ligation of murine left anterior descending coronary artery.Immmdiate timepoint of ligation procedure, autologous EPC from peripheral blood,positive for CD31,D34,Flk-1 and vWF,isolated from the peripheral blood of each animal were injected to ischemic myocardium.In the control groups,cell culture media was injected.Rats were euthanized and neovascularization in local ischemia areas was evaluated.Results Hematoxylin-eosin staining indicated better myocardial arrangement in the EPC group.Capillary density in ischemic myocardium weeks after transplantation was significantly greater in the group of EPC injection than the control groups.Conclusion Relatively purified EPC can be obtained by certain procedure of isolation and culture from rat peripheral blood.Intramyocardial transplantation of autologous EPCs will promote microangiogenesis and has a protective effect on ischemic myocardial tissue.