EXTRACTION AND STRUCTURAL STUDY OF SCHIZOPHYLLAN FROM SCHIZOPHYLLUM COMMUNE BY SUBMERGED CULTIVATION / 微生物学通报
Microbiology
; (12)1992.
Article
in Zh
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| ID: wpr-684315
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ABSTRACT
The extraction method and structure of exo-polysoccharide from schizophyllum commune by submerged cultivation were studied. Proteins were removed com p letely from the polysoccharide by the sevag method following the isoelectric poi nt precipitation method. The purified schizophyllan was proved to be homogeneou s with molecular weight 4?10 4D by sephadex G-200 column chromatography, PAGE and HPLC. Its monomer was determined by hydrolysis, PC, GC and its structure wa s analyzed by IR, enzymolysis, periodate oxidation, the results showed that schi zophyllan was only composed of glucose and it was the ?-glucan consisting of ?-(1-3) and ?-(1-6) glucosidic linkages.
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Language:
Zh
Journal:
Microbiology
Year:
1992
Type:
Article