FK228 blocks cells survival signal pathways and induces apoptosis of prostate cancer DU145 cells / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy
; (6)1995.
Article
in Zh
| WPRIM
| ID: wpr-684898
Responsible library:
WPRO
ABSTRACT
Objective:
To investigate the underlying mechanism of histone deacetylase (HDAC) inhibitor FK228-in- duced apoptosis of the prostate cancer cell line DU145.Methods:
The inhibitory effect of FK228 on DU145 cell growth and its cytotoxicity were determined by MTT assay;cell cycle arrest was detected by flow cytometry assay;morphological change was observed by Giemsa staining;and defined kinase protein levels were determined by Western blot analysis.Re- suitsFK228 obviously inhibited DU145 cells growth,arrested cell cycle at G_0/G_1 phase,induced cells morphological changes and degraded several kinase proteins,including EGFR,Her2,Raf-1,Src,Cdk4 and IAP member Survivin.The degradation of these kinases blocked Raf-Mek-Erk and PI3K/Akt survival signal pathways,inducing apoptosis.Condu- sionFK228 may induce DU145 cell apoptosis through depletion of multiple kinase proteins and blockade of survival sig- nal pathways of DU145 cells.
Full text:
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Cancer Biotherapy
Year:
1995
Type:
Article