Expression and Characterization of Thrombin Inhibitor TTI / 中国生物工程杂志
China Biotechnology
; (12)2006.
Article
in Zh
| WPRIM
| ID: wpr-686065
Responsible library:
WPRO
ABSTRACT
TTI gene coding for Tsetse thrombin inhibitor was modified with E.coli bias codon and expressed in Escherichia coli with high efficiency.Recombinant protein was purified to more than 98% purity.Assay for enzyme activity determination was set up.The result showed that the fusion protein exhibited inhibiting activity for thrombin.Inhibitory rate of purified TTI was 73% when concentration of thrombin and substrate was 10U/ml and 250?mol/L respectively.Inhibition pattern was determined as competitive with Ki at 35?mol/L.
Full text:
1
Index:
WPRIM
Language:
Zh
Journal:
China Biotechnology
Year:
2006
Type:
Article